The first step on the path to independence.

I know that this isn't exactly financial-related. Well, kinda tangentially, it is. Sure, yeah. Totally!

Anyway, off and on I've been toying with ideas for independent research projects. Independent here meaning my own research idea that I can start now and get some preliminary data that are also related enough to my boss's research to justify spending her money on the project but also something that she herself doesn't want to persue, so I could take it with me when I get a job. Basically, if I can get this started, get some sexy data, and write a great grant application, I can get a job. WOO!

Anyway, I've been thinking of ideas in whatever downtime I have for such thoughts. And I was toying with sticking in the polymerase field and possibly working on polymerases related to the one on which my boss (and I now) currently work. I hadn't nailed down anything specific, but I had a jist (a couple of proteins and a vague plan). Well, in the past month, I've stumbled upon some fabulously sexy results with my current polymerase, and at the same time, discovered that two other polymerases have the exact same structure and the same key conserved residues...these residues are conserved through practically every species that has these polymerases (human, mouse, chicken, dog, bacteria, virus, etc.). It's totally awesome.

So... BOOM! I have a concrete idea and a plan. And not 24 hours before a meeting with the boss where I propose said plan, she emails me and suggests that I do my plan. AWESOME! She had the same idea, so I know it's a good one.

Anyway, this past week, I've decided is the "official" start of my independent project. And it's totally exciting, because for one of my polymerases, I have to clone it, and this is actual cloning (cells -> RNA -> cDNA -> plasmid), which I've never done before. So, yeah, I'm totally pumped on this. So much so, that I've been designing my cloning strategy tonight at home. (Yes, I am that much of a nerd.)

So, while not completely relevant for this blog, it indicates that I'm a smidge closer to being able to get a real job and do real work. It's a great feeling! =)